Samba King returns for Johannesburg clash.
Brazil star Kaka returns from a one-match ban, while Elano has recovered from an ankle injury.
Robinho will also be available after missing the draw with Portugal because of muscular pains.
Felipe Melo is doubtful with a sprained ankle, while Julio Baptista is nursing a knee problem.
Chile will be without three players due to suspension.
Midfielder Marco Estrada was sent off against Spain, while defenders Waldo Ponce and Gary Medel received yellow cards, their second cautions of the tournament.
However, Matias Fernandez and Carlos Carmona return after bans.
Coach Marcelo Bielsa must decide whether to play target-man Humberto Suazo, who looks short of match fitness, or keep faith with Jorge Valdivia.
One booking from ban: Fabiano, Juan, Melo, Ramires (Brazil); Suazo, Valdivia (Chile) (BBC Sport)
South American sides have done very well thus far in South Africa and now two come face to face in Johannesburg for a game that could well turn out to be something of a classic. Chile have entertained many with their flowing attacking play with the likes of Alexis Sanchez really catching the eye.
Kaka returns for the Brazilians after their lifeless damp squib against Portugal and will be hoping to get his World Cup up and running after a fairly average personal performance at the finals.
Highlights of previous World Cup group matches these two have taken part in.
Chile 1-2 Spain
Portugal 0-0 Brazil