Reebok Stadium side will lose talisman until the start of next season.
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Bolton Wanderers midfielder Stuart Holden will miss the FA Cup semi-final with Stoke City after being ruled out of the next six months.
The 25-year-old suffered a knee injury in a challenge with Jonny Evans, who received a red card as a result, in the 1-0 defeat to Manchester United.
The United States international was taken immediately to hospital and underwent scans which have shown severe damage in the area.
This injury is the second serious problem Holden has sustained in his 14 months at the club. He broke his leg in March 2010 and only just returned before last summer’s World Cup.
Bolton manager Owen Coyle wishes his star player a speedy return to fitness and has praised the American’s contribution to this campaign.
“First and foremost our main concern is for Stuart and his recovery. He will get all the time and support that he needs,” Coyle told the club’s website.
“Stuart has been outstanding for me this season and he has shown in his performances that he is one of the best midfielders in the Barclays Premier League.
“He has such desire, strength of character and a winning mentality, and he will draw on those attributes in his rehabilitation.”