Old Trafford hero will miss Wembley showdown as FA upheld punishment for improper conduct.
Manchester United striker will miss the FA Cup semi-final with Manchester City after losing his appeal against a two-game ban.
The 25-year-old was charged with improper conduct after a foul-mouthed rant at a TV camera during the live 4-2 defeat of West Ham United.
The England international accepted the Football Association charge but appealed against the automatic ban as he felt it was excessive.
But the FA have ignored his pleas and apologies and stuck with the ban, meaning Rooney will miss crucial games in the season run-in.
He will have to watch Saturday’s home Premier League tie against Fulham as well as the all-Manchester FA Cup semi on April 16.
“A Regulatory Commission has suspended Manchester United’s Wayne Rooney for two matches,” read a statement issued by the FA.
“Rooney had admitted a charge for the use of offensive, insulting and/or abusive language, but claimed that the automatic penalty of two games was clearly excessive.
“The Commission did not accept the claim and Rooney will begin the standard two-match suspension with immediate effect.”