White Hart Lane club still do not agree with giving Upton Park side the Olympic Stadium.
Tottenham Hotspur are seeking a judicial review of West Ham United being awarded the Olympic Stadium and will take their case to the High Court.
This comes after the court last week rejected the legal claims from both Tottenham and League One side Leyton Orient.
Spurs will now present their case at a hearing. No-one from Orient was immediately available for a comment about their intentions.
West Ham were awarded the keys to the stadium last February before they were relegated from the Premier League last season.
A key aspect of their bid was to keep the running track around the pitch for it to be used to hold athletics meets in the capital after the games.
The Hammers will move in once they have modified the stadium to make it fit for purpose after London 2012.
“The club has today applied to the High Court to renew its application for permission to bring a claim against the London Borough of Newham and the Olympic Park Legacy Company, the Mayor of London and Government Ministers for judicial review of their decisions underlying the bid process for the conversion of the Olympic Stadium after the 2012 Games,” Tottenham said in a statement on their website.