Everton, Villa, Stoke and Sunderland represented, but Galaxy star Donovan misses out.
The United States of America cut their World Cup squad from 30 to 23 on Thursday night.
Los Angeles Galaxy forward Landon Donovan was the highest profile player to be omitted by coach Jurgen Klinsmann.
Four Premier League-based stars were included – Everton goalkeeper Tim Howard, Aston Villa stopper Brad Guzan, Stoke City defender Geoff Cameron and Sunderland striker Jozy Altidore.
Below is the squad in full.
— U.S. Soccer (@ussoccer) May 22, 2014
The USA have been drawn in Group G, along with Germany, Portugal and Ghana.